Journal: ImmunoHorizons

Article Title: Chemokine Receptor CCR2 Is Protective toward Outer Hair Cells in Chronic Suppurative Otitis Media

doi: 10.4049/immunohorizons.2400064

Figure Lengend Snippet: CCR2 deletion leads to increased OHC loss in CSOM. Representative whole-mount sections of the cochlea (basal, middle) in CSOM at 10 d from CCR2 +/+ CSOM ( A and B ) and CCR2 −/− ( C and D ) mice. Arrows show OHC loss. ( E ) The OHC survival rate as a percentage in the cochlear basal turn of both groups (* p = 0.036). Red, myosin VIIa. The number of mice per group is shown in brackets. Data are shown as mean ± SD. Scale bar, 100 μm.

Article Snippet: To check BLB permeability in CCR2 −/− CSOM, FITC-dextran (MilliporeSigma, 46944-100MG-F) was injected i.v. Each mouse was administrated 200 μl at the concentration of 5 μg/μl.

Techniques:

Journal: ImmunoHorizons

Article Title: Chemokine Receptor CCR2 Is Protective toward Outer Hair Cells in Chronic Suppurative Otitis Media

doi: 10.4049/immunohorizons.2400064

Figure Lengend Snippet: Macrophages are reduced in CCR2 −/− CSOM. ( A – F ) Cryosections of the cochlea (basal, middle, and apical turn) are shown in CCR2 +/+ CSOM (left) and CCR2 −/− CSOM (right) mice at 10 d following middle ear infection. ( G ) A significant decrease in the number of macrophages per turn (*** p < 0.001). The number of mice per group is shown in parentheses. The data represent mean ± SD. Green, F4/80 (arrows). Scale bar, 100 µm.

Article Snippet: To check BLB permeability in CCR2 −/− CSOM, FITC-dextran (MilliporeSigma, 46944-100MG-F) was injected i.v. Each mouse was administrated 200 μl at the concentration of 5 μg/μl.

Techniques: Infection

Journal: iScience

Article Title: NLRP6 induces RIP1 kinase-dependent necroptosis via TAK1-mediated p38 MAPK /MK2 phosphorylation in S . typhimurium infection

doi: 10.1016/j.isci.2024.109339

Figure Lengend Snippet: NLRP6 deficiency decreases S . typhimurium -induced enterocyte necroptosis and intestinal barrier dysfunction (A) Cell death of WT and NLRP6 −/− Caco-2 cells treated with vehicle, NSA or GSK’872 for 1 h, followed by infection with S . typhimurium strain SL1344 at an MOI of ∼100 for 4 h. (B) Western blot analysis and relative intensity of WT and NLRP6 −/− Caco-2 cells infected with SL1344 at an MOI of ∼100 for 4 h. (C) Cell death of WT and NLRP6−/− Caco-2 cells treated with vehicle, zVAD or zVAD + Nec-1 for 1 h, followed by infection with S. typhimurium strain SL1344 at an MOI of ∼100 for 4 h. (D and E) Western blot analysis and relative intensity of WT and NLRP6 −/− Caco-2 cells infected with SL1344 at an MOI of ∼100 for 4 h. (F) Cell death of WT and NLRP6 −/− Caco-2 cells treated with vehicle or (5Z)-7-Oxozeaenol for 1 h, followed by infection with SL1344 at an MOI of ∼100 for 4 h. (G) Cell death of WT and NLRP6 −/− Caco-2 cells treated with vehicle or SB239063 for 30 min, followed by infection with SL1344 at an MOI of ∼100 for 4 h. (H) Western blot analysis and relative intensity of WT and NLRP6 −/− Caco-2 cells infected with SL1344 at an MOI of ∼100 for 4 h. (I) Cell death of WT and NLRP6 −/− Caco-2 cells treated with vehicle or MK2-IN-1 for 30 min, followed by infection with SL1344 at an MOI of ∼100 for 4 h. (J) Western blot analysis and relative intensity of WT and NLRP6 −/− Caco-2 cells infected with SL1344 at an MOI of ∼100 for 4 h. (K) TEER levels of WT and NLRP6 −/− Caco-2 cell monolayers infected with SL1344 at an MOI of ∼100. (L) FD4 permeability of WT and NLRP6 −/− Caco-2 cell monolayers infected with SL1344 at an MOI of ∼100 for 3 h. (M) Bacterial translocation of WT and NLRP6 −/− Caco-2 cell monolayers infected with SL1344 at an MOI of ∼100 for 3 h. Data were compared with independent Student’s t test. Values are expressed as the mean ± SEM, and statistically significant differences are indicated. All data are representative of at least three independent experiments. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001; ns, not significant.

Article Snippet: Epithelial permeability was measured by apical-to-basal transport of FD4 (46944, MilliporeSigma).

Techniques: Infection, Western Blot, Permeability, Translocation Assay

Journal: iScience

Article Title: NLRP6 induces RIP1 kinase-dependent necroptosis via TAK1-mediated p38 MAPK /MK2 phosphorylation in S . typhimurium infection

doi: 10.1016/j.isci.2024.109339

Figure Lengend Snippet:

Article Snippet: Epithelial permeability was measured by apical-to-basal transport of FD4 (46944, MilliporeSigma).

Techniques: Virus, Recombinant, Modification, Lysis, Lactate Dehydrogenase Assay, Cell Viability Assay, Bradford Protein Assay, Luciferase, Reporter Assay, Western Blot, Sequencing, Control, Plasmid Preparation, Expressing, Software